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Title 

Control of direct and indirect somatic embryogenesis by exogenous growth regulators in immature zygotic embryo cultures of rose

Authors 

Suk Weon KimSeung Chul OhJang Ryol Liu

Publisher 

Springer Verlag (Germany)

Issue Date 

2003

Citation 

Plant Cell Tissue and Organ Culture, vol. 74, no. 1, pp. 61-66

Keywords 

embryogenic callusimmature zygotic embryoplant regenerationrosa hybridasomatic embryogenesisplant cell culture

Abstract 

Immature zygotic embryos of rose (Rosa hybrida L.; cv. Sumpath) did not form somatic embryos or embryogenic calluses when cultured on half-strength Murashige and Skoog's medium supplemented with various concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) as the sole growth regulator. However, the zygotic embryos produced somatic embryos without an intervening callus phase at a frequency of 27.3% on medium with 4.44 μM 6-benzyladenine (BA) alone. Immature zygotic embryos formed embryogenic calluses at a frequency of 25% on medium with a combination of 1.36 μM 2,4-D and 4.44 μM BA. Upon transfer to medium without growth regulators, embryogenic calluses produced numerous somatic embryos that subsequently developed into plantlets. Somatic embryos were induced directly from immature zygotic embryos, or indirectly via an intervening callus phase, by manipulating the exogenous growth regulators. Plantlets were successfully transplanted to potting soil and grown to maturity in a greenhouse.

ISSN 

0167-6857

Link 

http://dx.doi.org/10.1023/A:1023355729046

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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