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Title 

A Novel approach to isolation and functional characterization of genomic DNA sequences from the methylotrophic yeast Hansenula polymorpha

Authors 

M O AgaphonovA V DeevS Y KimJung Hoon SohnEui Sung ChoiM D Ter-Avanesyan

Publisher 

Springer Verlag (Germany)

Issue Date 

2003

Citation 

Molecular Biology +, vol. 37, no. 1, pp. 74-80

Keywords 

hansenula polymorphaintronprotein secretionrecombinationsplicingvacuolecarboxypeptidase CDNA fragmentfungal proteingenomic DNA

Abstract 

A novel approach to isolation and functional characterization of the Hansenula polymorpha genes basing on the use of two strains of different origin is described. One of these strains is better suited for the isolation of genomic DNA fragments, while the other is preferable for their functional analysis. Thirty three genomic sequences governing expression of a reporter protein have been isolated. Analysis of the sequence encoding a homolog of the Saccharomyces cerevisiae cofilin revealed two introns. Another isolated DNA fragment encoded a homolog of the S. cerevisiae Vps10p. Disruption of the corresponding gene resulted in secretion of a vacuolar protein, carboxypeptidase Y, into the culture medium.

ISSN 

0026-8933

Link 

http://dx.doi.org/10.1023/A:1022336914110

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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