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Title 

Plant regeneration of rose (Rosa hybridia) from embryogenic cell-derived protoplasts

Authors 

Suk Weon KimSeung Chul OhD S InJang Ryol Liu

Publisher 

Springer Verlag (Germany)

Issue Date 

2003

Citation 

Plant Cell Tissue and Organ Culture, vol. 73, no. 1, pp. 15-19

Keywords 

myo-inositoprotoplast culturesomatic embryogenesisplant cell cultureplant regenerationplants (botany)cellsplantsrosarosa hybrid cultivar

Abstract 

Culture conditions are described for sustained cell division and plant regeneration from protoplasts of rose (Rosa hybrida L. 'Sumpath'). Protoplasts were enzymatically isolated from 2-week-old embryogenic cell suspension cultures. Freshly isolated protoplasts were plated as a thin layer onto protoplast culture medium (half-strength Murashige and Skoog's medium containing 60 g l-1 myo-inositol, 4.4 μM BA, and 1.4 μM 2,4-D) at a density of 5×104 protoplasts ml-1. The plating efficiency reached 3.9% after 2 weeks of culture. However, few protoplasts underwent cell division when cultured in protoplast culture medium in which 60 g l-1 myo-inositol was replaced with the same osmolarity of 90 g l-1 mannitol, indicating that myo-inositol is essential for sustained cell division of protoplasts. Colonies were formed after 8 weeks of culture at a frequency of 0.2%. Colonies were then transferred to colony culture medium (0.4% Gelrite-solidified protoplast culture medium) and maintained by subculturing at 4-week intervals to form embryogenic calluses. Upon transfer to half-strength MS basal medium, embryogenic calluses gave rise to numerous somatic embryos. Somatic embryos were transferred to half-strength MS basal medium containing 48 mg l-1 ferric ethylenediamine di-(o-hydroxyphenylacetate), where they subsequently developed into plantlets at a frequency of 30.9%. The plantlets had the same chromosome number of 2n=3x=21 as the source plant. They were successfully transplanted to potting soil and grown to maturity in a greenhouse.

ISSN 

0167-6857

Link 

http://dx.doi.org/10.1023/A:1022693605436

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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