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Title 

First-order kinetics analysis of monomer composition dependent polyhydroxyalkanoic acid degradation in Pseudomonas spp.

Authors 

M H ChoiJ K RhoH J LeeJae Jun SongS C YoonS Y Lee

Publisher 

American Chemical Society

Issue Date 

2003

Citation 

Biomacromolecules, vol. 4, no. 2, pp. 424-428

Keywords 

organic acidsreaction kineticsfirst order kineticsmonomersdecanoic aciddecanoic acid derivativehexanoic acid derivativemonomernonanoic acidoctanoic acid

Abstract 

The intracellular degradation of polyhydroxyalkanoic acid (PHA) in pseudomonads was investigated by first-order kinetics analysis using the initial rate method. One type of PHA was accumulated in five Pseudomonas spp., P. oleovorans, P. aeruginosa, P. fluorescens, P. citronellolis, and P. putida, by growing them on octanoic acid. The monomer compositions of the five PHA were not significantly different from one another: 85-90 mol % 3-hydroxyoctanoic acid (3HO), 7-12 mol % 3-hydorxycaproic acid (3HC), and 3-6 mol % 3-hydroxydecanoic acid (3HD). The first-order degradation rate constants (k1) for the octanoate-derived PHA (designated P(3HO)) in the five species were in a similar range between 0.060 and 0.088 h-1. This may indicate the similar specificities of the five intracellular depolymerases. In addition, the similar k1 among the different species may correlate with the high degree of amino acid sequence identities (over 85 %) among the intracellular PHA depolymerase phaZ genes. Six other chemically different types of PHA were accumulated in P. putida from n-nonanoic acid, n-decanoic acid, 5-phenyvaleric acid, or 11-phenoxyundecanoic acid as a single or a mixed carbon source. The calculated k1 values were characteristic to each PHA, reflecting their chemical structures. In comparison with P(3HO), an increase in the levels of the two minor monomers 3HC and 3HD as in P(21 mol % 3HC-co-56 mol % 3HO-co-23 mol % 3HD) significantly slowed the rate of intracellular degradation. From the comparison of k1 values, it is suggested that the P. putida intracellular depolymerase is most active against P(3HO).

ISSN 

1525-7797

Link 

http://dx.doi.org/10.1021/bm0257199

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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