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Title 

Overexpression of thermoalkalophilic lipase from Bacillus stearothermophilus L1 in Saccharomyces cerevisiae

Authors 

Jung Oh AhnH W JangHong-Weon LeeEui Sung ChoiS J HaamTae Kwang OhJoon Ki Jung

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

2003

Citation 

Journal of Microbiology and Biotechnology, vol. 13, no. 3, pp. 451-456

Keywords 

bacillus stearothermophilus L1induction timesaccharomyces cerevisiaethermoalkalophilic lipaselipoprotein lipasegene overexpressiongeobacillus stearothermophilussaccharomyces

Abstract 

An expression vector system was developed for the secretory production of recombinant Bacillus stearothermophilus L1 lipase in Saccharomyces cerevisiae. The mature L1 lipase gene was fused to α-amylase signal sequence from Aspergillus oryzae for the effective secretion into the culture broth and the expression was controlled under GAL10 (the gene coding UDP-galactose epimerase of S. cerevisiae) promoter. S. cerevisiae harboring the resulting plasmid successfully secreted L1 lipase into the culture broth. To examine an optimum condition for L1 lipase expression in the fed-batch culture, L1 lipase expression was induced at three different growth phases (early, mid, and late-exponential growth phases). Maximum production of L1 lipase (1,254,000 U/l, corresponding to 0.65/l) was found when the culture was induced at an early growth phase. Secreted recombinant L1 lipase was purified only through CM-Sepharose chromatography, and the purified enzyme showed 1,963 U/mg of specific activity and thermoalkalophilic properties similar to those reported for the enzyme expressed in Escherichia coli.

ISSN 

1017-7825

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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