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Title 

Separation and purification of teicoplanin by diaion HP-20 and concanavalin A chromatography

 

소수성 diaion HP-20 및 친화성 concanavalin A 크로마토그래피를 이용한 glycopeptide계 항생제 teicoplanin의 분리 및 정제

Authors 

Jae-Chan LeeHae Ryong ParkDong Jin parkY B KimChang-Jin Kim

Publisher 

The Korean Society for Applied Microbiology and Biotechnology

Issue Date 

2003

Citation 

Korean Journal of Microbiology & Biotechnology, vol. 31, no. 2, pp. 201-204

Keywords 

actinoplanes teicomyceticusconcanavalin Adiaion HP-20teicoplaninantibiotic agentsugaradsorptionaffinity chromatographybacterial growthbacterial strain

Abstract 

Glycopeptide antibiotics, teicoplanin was purified from a mutant strain of Actinoplanes teichomyceticus ATCC31121, A. teichomyceticus MSL2211. We developed a simple procedure to separate and purify the teicoplanin from the fermentation broth. Teicoplanin was purified by two-step purification system, hydrophobic adsorption and sugar affinity chromatography in combination with HPLC analysis based on the properties of hydrophobic acyl chain and sugar moiety in teicoplanin. Teicoplanin was separated from the culture broth by Diaion HP-20 and further purified by concanavalin A affinity column chromatography. As an adsorbent resin, Diaion HP-20 in broth eliminated toxic effects on growth, reduced feedback repression of teicoplanin production, and assisted in rapid recovery of teicoplanin. The teicoplanin displayed the final yield of 80% and 95% of purity.

ISSN 

0257-2389

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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