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Title 

Gentiobiose synthesis from glucose using recombinant β-glucosidase from Thermus caldophilus GK24

Authors 

T Y KimDae Sil LeeH J Shin

Publisher 

Springer Verlag (Germany)

Issue Date 

2003

Citation 

Biotechnology and Bioprocess Engineering, vol. 8, no. 0, pp. 210-212

Keywords 

β-glucosidasegentiobiosethermus caldophilus GK24

Abstract 

Recombinant β-glucosidase from Thermus caldophilus GK24 was easily purified partially by a heat treatment procedure, resulting in 8-fold and recovery yield of 80% from crude enzyme. When the β-glucosidase was incubated with a 80% glucose solution (w/w), gentiobiose (β1,6-glucobiose) was the major product in the reaction mixture. The optimal conditions for producing gentiobiose (11% yields of total sugar) were pH 8-9 and 70°C for 72 h.

ISSN 

1226-8372

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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