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Title 

Analysis of plausible downstream target genes of Hoxc8 in F9 teratocarcinoma cells : Putative downstream target genes of Hoxc8

Authors 

Y J KwonJeong Heon KoB K KimM H Kim

Publisher 

Springer Verlag (Germany)

Issue Date 

2003

Citation 

Molecular Biology Reports, vol. 30, no. 3, pp. 141-148

Keywords 

murine Hoxc8 geneproteom analysisputative Hoxc8 realizator geneshoxc8 proteinanimal cellgene controlgene overexpressiongene targetingteratocarcinomacell line, tumor

Abstract 

Although Hox genes are known to mediate developmental decisions involved in pattern formation during embryogenesis, it is still not well understood what Hox regulates. In order to analyze Hoxc8 downstream target genes, a stable cell line overexpressing Hoxc8 was established using F9 murine teratocarcinoma cells, proteom samples were analyzed by 2-DE, and compared with controls. The protein spots having differences more than 4 fold in intensity were selected, analyzed by MALDI-TOF, and grouped in terms of putative function; cytoskeleton and motility (vimentin, γ-actin, tropomyosin, and tubulin β-5 chain); folding, modification and degradation of protein (GRP78, proteasome subunit α type 5, 26S proteasome regulatory subunit p27 protein, and PDIR); metabolism (ATP synthase beta subunit, Pgam1, and CAII); transcription/translation factors and general nucleic acid binding proteins (RbAp46, PCNA, eEF-1-beta, and nucleophosmin). Although it may not be significant, 50% of the genes were located on chromosomes 2 and 3, suggesting the possibility of a non-random distribution of Hox downstream genes. Almost 50% of the genes analyzed showed some relation with Hox protein directly or indirectly; i.e., tubulin beta 5, EF-1 beta and PCNA have been reported to contain putative Hox binding regulatory sites and genes like vimentin, pgam1 and nucleophosmin to be regulated by RA, a potent modulator of Hox expression. These results altogether imply that proteom analysis could be a possible tool for the analysis of the potent Hox realizator genes, which provides a new insight into the function of Hox on pattern formation during embryogenesis.

ISSN 

0301-4851

Link 

http://dx.doi.org/10.1023/A:1024920418148

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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