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Title 

Discovery of genes for ginsenoside biosynthesis by analysis of ginseng expressed sequence tags

Authors 

J D JungH W ParkY HahnCheol-Goo HurD S InH J ChungJang Ryol LiuD W Choi

Publisher 

Springer Verlag (Germany)

Issue Date 

2003

Citation 

Plant Cell Reports, vol. 22, no. 3, pp. 224-230

Keywords 

expressed sequence tagsginsenosideoxidosqualene cyclasepanax ginsengsecondary metabolismbiosynthesisgenesexpressed sequence tags (EST)expressed sequence tag

Abstract 

Expressed sequence tags (ESTs) provide a valuable tool that can be used to identify genes in secondary metabolite biosynthesis. Ginseng (Panax ginseng C.A Meyer) is a medicinal plant that accumulates ginsenosides in roots. We sequenced 11,636 ESTs from five ginseng libraries in order to create a gene resource for biosynthesis of ginsenosides, which are thought to be the major active component in roots. Only 59% of the ginseng ESTs exhibited significant homology to previously known polypeptide sequences. Stress- and pathogen-response proteins were most abundant in 4-year-old ginseng roots. ESTs involved in ginsenoside biosynthesis were identified by a keyword search of BLASTX results and a domain search of ginseng ESTs. We identified 4 oxidosqualene cyclase candidates involved in the cyclization reaction of 2,3-oxidosqualene, 9 nine cytochrome P450 and 12 glycosyltransferse candidates, which may be involved in modification of the triterpene backbone.

ISSN 

0721-7714

Link 

http://dx.doi.org/10.1007/s00299-003-0678-6

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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