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Title 

The T cell receptor β enhancer promotes access and pairing of Dβ and Jβ gene segments during V(D)J recombination

Authors 

Chun Jeih RyuB B HainesD D DraganovYun Hee KangC E WhitehurstT SchmidtHyo Jeong HongJ Chen

Publisher 

National Academy of Sciences

Issue Date 

2003

Citation 

Proceedings of the National Academy of Sciences of the United States of America, vol. 100, no. 23, pp. 13465-13470

Keywords 

CD4 antigenCD8 antigenelementT lymphocyte receptor beta chainanimal cellcontrolled studyenhancer regiongene inactivationgene rearrangementgenetic recombination

Abstract 

The precise function of cis elements in regulating V(D)J recombination is still controversial. Here, we determined the effect of inactivation of the TCRβ enhancer (Eβ) on cleavage and rearrangement of Dβ1, Dβ2, Jβ1, and Jβ2 gene segments in CD4-CD8 - [double-negative (DN)] and CD4+CD8+ [double-positive (DP)] thymocytes. In Eβ-deficient mice, (i) Dβ1 rearrangements were more severely impaired than Dβ2 rearrangements; (ii) most of the Dβ and Jβ cleavages and rearrangements occurred in DP, rather than in DN, thymocytes; and (iii) most of the 3′ Dβ1 cleavages were coupled to 5′ Dβ2 cleavages instead of to Jβ cleavages, resulting in nonstandard Dβ1-Dβ2-Jβ2 joints. These findings suggest that the Eβ regulates TCRβ rearrangement by promoting accessibility of Dβ and Jβ gene segments in DN thymocytes and proper pairing between Dβ1 and Jβ gene segments for cleavage and joining in DP thymocytes.

ISSN 

0027-8424

Link 

http://dx.doi.org/10.1073/pnas.2235807100

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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