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Title 

Scale-up of recombinant hirudin production from Saccharomyces cerevisiae

Authors 

Chul Ho KimK J RaoD J YounSang Ki Rhee

Publisher 

Springer Verlag (Germany)

Issue Date 

2003

Citation 

Biotechnology and Bioprocess Engineering, vol. 8, no. 5, pp. 303-305

Keywords 

fed batch fermentationGAL promoterrecombinant hirudinsaccharomyces cerevisiaescale-up

Abstract 

Scale-up of hirudin production from Saccharomyces cerevisiae from bench-scale to pilot-scale was carried out based on constant volumetric oxygen transfer coefficient (KLa). Fed-batch mode of cultivation using step-wise feeding strategy of galactose was employed for the production of hirudin in a 30-L and a 300-L pilot-scale fermentor. The final hirudin concentrations were achieved 390 mg/L and 286.1 mg/L, and the volumetric productivities were 80.4% and 90.7% with the 30-L and 300-L fermentors, respectively, compared to the productivity of the 5-L bench-scale fermentor.

ISSN 

1226-8372

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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