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Title 

Characterization of a human WNT7B gene and its up regulation in gastric cancer cell lines

Authors 

Jeong Min KimHo Yong SohnJung Hwa OhJ G KimNam-Soon KimYong Sung Kim

Publisher 

Korean Society for Brain and Neural Science

Issue Date 

2003

Citation 

Experimental Oncology, vol. 25, no. 3, pp. 211-215

Keywords 

expression profilesfull-length cDNAgastric cancerhuman WNT7b genetumorigenesiscomplementary DNARNAasparagine derivativeprotein Wnt7bunclassified drug

Abstract 

The WNT family of secreted proteins is a group of signaling molecules that control a diverse range of developmental processes including cell proliferation and tumorigenesis. We have constructed a subtracted full-length cDNA library from a human gastric cell line SNU5 using the capping and subtraction method and isolated and characterized the full-length cDNA of human WNT7b. Cloned cDNA sequence comprised 1440 bp including the 5′-noncoding region of 374 bp, an ORF of 1050 bp and the 3′-noncoding region of 16 bp. The ORF could encode a protein of 349 amino acids. Analysis of deduced amino acid sequence showed a conserved WNT signature sequence (CKCHGvSGSC), characteristic 25 cysteine residues and three different N-glycosylation sites at 83, 127, and 295 of asparagines. Comparison of cloned WNT7b with previously reported human WNT7b revealed that cloned WNT7b has 100% identity with nucleotide sequence in coding sequence but has more 279 bp than that in 5′-noncoding region. Also, comparison of cloned WNT7b with mouse WNT7b showed 91% identity in nucleotide sequence and 99% in amino acid sequence. Furthermore, a phylogeny of WNT7b in relation to other known WNT proteins revealed that human WNT7b has a close paralog with other WNT7b proteins across the different species and genus, not with human WNT7a. Besides, expression profiles in different gastric cell lines by RT-PCR showed higher expression of human WNT7b in cancer cell lines than in normal cells, suggesting that human WNT7b has a crucial role in gastric tumorigenesis.

ISSN 

0204-3564

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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