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Title 

Transgenic ginseng cell lines that produce high levels of a human lactoferrin

Authors 

Suk Yoon KwonSeung Hyun JoOk Sun LeeSun Mee ChoiSang Soo KwakHaeng Soon Lee

Publisher 

Georg Thieme Verlag

Issue Date 

2003

Citation 

Planta Medica, vol. 69, no. 11, pp. 1005-1008

Keywords 

araliaceaehigh expression promoterhuman lactoferrinpanax ginsengrecombinant proteintransgenic cell culturescomplementary DNAlactoferrinsignal peptidecell culture

Abstract 

In order to produce a human lactoferrin (hLf) protein in cultured plant cells, we developed Korean ginseng (Panax ginseng) cell line using an oxidative stress-inducible peroxidase (SWPA2) promoter and characterized the production of human lactoferrin in cultured cells. A construct containing a targeting signal peptide from tobacco endoplasmic reticulum fused to human lactoferrin cDNA under the control of SWPA2 promoter was engineered. Transgenic Korean ginseng cell lines that produced a recombinant hLf protein were successfully generated and confirmed by PCR and Southern blot analyses. Western blot and ELISA analyses showed that hLf protein was synthesized in the transgenic cells. The production of hLf showed a maximal level (up to 3.0% of total soluble protein) in the stationary phase of callus cultures. These results suggest that the transgenic cell lines in this study will be biotechnologically useful for the commercial production of hLf protein in cell cultures, with no need for purification.

ISSN 

0032-0943

Link 

http://dx.doi.org/10.1055/s-2003-45146

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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