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Title 

Stability of murine, chimeric and humanized antibodies against pre-S2 surface antigen of hepatitis B virus

Authors 

Sung Sup ParkJ H KimJ F BrandtsHyo Jeong Hong

Publisher 

Elsevier

Issue Date 

2003

Citation 

Biologicals, vol. 31, no. 4, pp. 295-302

Keywords 

humanized antibodymicrocalorimetryPre-S2stabilitychimeric antibodyhepatitis B antigenimmobilized antibodymonoclonal antibodyrecombinant antibodyantibody combining site

Abstract 

We have constructed a humanized antibody with specificity for the pre-S2 surface antigen of hepatitis B virus (HBV) by grafting the complementarity determining regions (CDRs) of parental murine monoclonal antibody (mAb) into human anti-Sm antibody framework regions. The humanized antibody has a substitution at position 94 in a framework region of the heavy chain variable region, and exhibits the same antigen binding affinity as the parental murine monoclonal and chimeric antibodies. In order to assess the stability of these antibodies, thermal inactivation of the parental, chimeric and humanized antibodies was analyzed. Fifty percent inactivation of the chimeric and humanized antibodies was observed at 63.7°C and 68.7°C, respectively, compared to 55.0°C for murine antibody. The humanized antibody also exhibited increased stability against denaturant. Guanidine-induced unfolding monitored by the changes in fluorescence intensity at 360 nm showed that midpoints of the transition of the chimeric and humanized antibodies were 2.47 M and 2.56 M, respectively, whereas that of the murine antibody was 1.36 M.

ISSN 

1045-1056

Link 

http://dx.doi.org/10.1016/j.biologicals.2003.08.003

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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