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Title 

Independent exponential feeding of glycerol and methanol for fed-batch culture of recombinant Hansenula polymorpha DL-1

Authors 

H MoonS W KimJ LeeSang Ki RheeEui Sung ChoiHyun Ah KangI H KimS I Hong

Publisher 

Humana Press

Issue Date 

2003

Citation 

Applied Biochemistry and Biotechnology, vol. 111, no. 2, pp. 65-80

Keywords 

exponential feedingfed-batch culturehansenula polymorphahuman epidermal growth factormixed feedglycerolmethanolfed batch cultureculture media

Abstract 

As a novel feeding strategy for optimizing human epidermal growth factor (hEGF) production with a recombinant Hansenula polymorpha DL-1 using the methanol oxidase (MOX) promoter in H. polymorpha DL-1, independent exponential feeding of two substrates was used. A simple kinetic model considering the cell growth on two substrates was established and used to calculate the respective feeding rates of glycerol and methanol. In the fed-batch culture with methanol-only feeding, the optimal set point of specific growth rate on methanol was found to be 0.10 h-1. When the fed-batch cultures were conducted by the independent feeding of glycerol and methanol, the actual specific growth rate on glycerol and methanol was slightly lower than the set point of specific growth rate. By the uncoupled feeding of glycerol and methanol the volumetric productivity of hEGF increased from 6.4 to 8.0 mg/(L·h), compared with methanol-only feeding.

ISSN 

0273-2289

Link 

http://dx.doi.org/10.1385/ABAB:111:2:65

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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