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Title 

Differentiation of actinomycete genera based on partial rpoB gene sequences

 

rpoB 유전자 부분 분석에 의한 방선균 속 동정

Authors 

B J KimY H KohJ S ChunChang-Jin KimS H LeeM J ChoJ W HyunK H LeeC Y ChaY H Kook

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

2003

Citation 

Journal of Microbiology and Biotechnology, vol. 13, no. 6, pp. 846-852

Keywords 

actinomycete generadifferentiationidentificationphylogenetic relationshiprpoBsignature amino acidsamino acid sequencegene constructphylogenetic treesequence analysis

Abstract 

rpoB DNAs (279 bp) from 34 species of 5 actinomycete genera were sequenced and a phylogenetic tree was constructed based on the sequences obtained. The genera were clearly differentiated in the rpoB tree, forming clades specific to their respective genus. In addition, 2 signature amino acid residues specific to Streptomyces were found in a multiple alignment of the deduced amino acid sequences. To empirically confirm that this rpoB gene analysis system could be used to differentiate actinomycete isolates, the proposed system was used to identify 16 actinomycete isolates from Jeju Island. All isolates were successfully differentiated into the genera Streptomyces and Micromonospora. Accordingly, this is the first report that an rpoB sequence analysis has been effectively used to differentiate actinomycete strains at the genus level.

ISSN 

1017-7825

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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