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Title 

Isolation and characterization of a Lymantria dispar multinucleocapsid nucleopolyhedrovirus isolate in Korea

Authors 

H J ShimJ Y RohJ Y ChoiM S LiS D WooHyun Woo OhK S BooY H Je

Publisher 

Microbiological Society of Korea

Issue Date 

2003

Citation 

Journal of Microbiology, vol. 41, no. 4, pp. 306-311

Keywords 

baculovirusenhancinLdMNPV-NMlymantria disparamino acid sequenceenhancin geneKorealarvanonhumanprotein determination

Abstract 

In Korea, a Lymantria dispar multinucleocapsid nucleopolyhedrovirus, LdMNPV-NM, was isolated and characterized from dead L. dispar larvae. The polyhedra of LdMNPV-NM were irregularly shaped with a diameter of 1.62±0.33 μm. Numerous virions comprised of the multinucleocapsid were evident in the electron microscopic examination of the polyhedra cross sections. These polyhedra were composed of a major protein of 30 kDa. The restriction enzyme digestion patterns of LdMNPV-NM showed that this isolate had some different fragments from those of the Gypchek® LdMNPV isolate, although their overall profiles were similar. The deduced amino acid sequence of the enhancin gene of LdMNPV-NM showed differences when compared to previously reported enhancin genes of other LdMNPV strains. These results suggested that the LdMNPV-NM isolate from Korea was a new NPV strain and had a new enhancin gene.

ISSN 

1225-8873

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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