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Title 

Mad1p, a component of the spindle assembly checkpoint in fission yeast, suppresses a novel septation-defective mutant, sun1, in a cell-division cycle

Authors 

I G KimD K RheeJ W JeongS C KimMi Sun WonJ H LeeK W SongH B Kim

Publisher 

Wiley-Blackwell

Issue Date 

2003

Citation 

FEMS Microbiology Letters, vol. 227, no. 2, pp. 183-188

Keywords 

cell-division cyclecytokinesismad1pschizosaccharomyces pombespindle checkpointactinfungal proteinmutant proteinprotein Mad1unclassified drug

Abstract 

To enhance our understanding of the cytokinesis, we have carried out a genetic screen for temperature-sensitive Schizosaccharomyces pombe mutants that show defects in septum formation and cell division. Here we present the isolation and characterization of a new temperature-sensitive mutant, sun1 (septum uncontrolled), which undergoes uncontrolled septation during cell-division cycle at restrictive temperature (37°C). In sun1 mutant, the actin ring and septum are positioned at random locations and angles, and the nuclear division cycle continues. These observations suggest that the sun1 gene product is required for the proper placement of the actin ring as well as precise septation. In a screen for the sun1+ gene to complement the sun1 mutant, we have isolated a mad1+ (mitotic arrest deficient) gene, which encodes a component of the spindle checkpoint in the cell-division cycle. Analysis of crossing the sun1 cell with the mad1+ null mutant indicates that mad1+ suppresses the sun1 mutant defective in controlled septation in a cell-division cycle.

ISSN 

0378-1097

Link 

http://dx.doi.org/10.1016/S0378-1097(03)00607-4

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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