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Title 

Costunolide inhibits interleukin-1β expression by down-regulation of AP-1 and MAPK activity in LPS-stimulated RAW 264.7 cells

Authors 

Jong Soon KangYeo Dae YoonKi Hoon LeeSong Kyu ParkHwan Mook Kim

Publisher 

Elsevier

Issue Date 

2004

Citation 

Biochemical and Biophysical Research Communications, vol. 313, no. 1, pp. 171-177

Keywords 

AP-1costunolideinflammationinterleukin-1βMAP kinase1,4 diamino 1,4 bis(2 aminophenylthio) 2,3 dicyanobutadieneanthra[1,9 cd]pyrazol 6(2h) oneinterleukin 1betatranscription factor AP 1

Abstract 

Costunolide, a sesquiterpene lactone isolated from the root of Saussurea lappa Clarke, is known to have a variety of biological activities, including anti-carcinogenic and anti-fungal activities. Here, we demonstrated the inhibitory effect of costunolide on the protein and mRNA expression of interleukin-1β (IL-1β) in LPS-stimulated RAW 264.7 cells. We also showed that costunolide suppressed the transcriptional activity of the IL-1β promoter. Moreover, costunolide inhibited the activity of AP-1 transcription factor, and the phosphorylation of MAPKs, including SAPK/JNK and p38 MAP kinase. The inhibitory effect of costunolide on AP-1 activity was also confirmed by an electrophoretic mobility shift assay. Additionally, specific inhibitors of SAPK/JNK and p38 MAP kinase, SP600125 and SB203580, also suppressed LPS-induced increase in IL-1β gene expression and AP-1 DNA binding. Taken together, these results demonstrate that costunolide inhibits IL-1β gene expression by blocking the activation of MAPKs and DNA binding of AP-1 in LPS-stimulated RAW 264.7 cells.

ISSN 

0006-291X

Link 

http://dx.doi.org/10.1016/j.bbrc.2003.11.109

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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