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Title 

Evidence of interaction of phage P22 tailspike protein with DnaJ during translational folding

Authors 

Sang Chul LeeM H Yu

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

2004

Citation 

Journal of Microbiology and Biotechnology, vol. 14, no. 1, pp. 162-166

Keywords 

bacterophage P22DnaJmolecular chaperoneprotein foldingtailspike proteinheat shock proteinprotein DnaJprotein DnaKvirus proteinbacteriophage

Abstract 

Phage P22 tailspike is a thermostable homotrimeric protein, and temperature-sensitive folding (tsf) and global suppressor mutations affect its folding yields at elevated temperatures. We earlier suggested that the folding of the tailspike protein in Escherichia coli requires an unidentified molecular chaperone. Accordingly, in the present study, the interactions of purified DnaK, DnaJ, and GrpE heat-shock proteins with the tailspike protein were investigated during the translation and folding of the protein. The cotranslational addition of DnaJ to the tailspike protein resulted in the arrest of folding, when Dnak and GrpE were missing. However, the presence of DnaK, DnaJ, and GrpE had no effect on the folding yield of the tailspike protein, thus, providing evidence for the binding of the nascent tailspike protein with DnaJ protein, a member of DnaK chaperoning cycle.

ISSN 

1017-7825

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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