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Title 

Detection of Nocardia sp. H17-1 by PCR during bioremediation of crude oil-contaminated soil

 

원유 오염토양의 bioremediation 과정 동안 PCR을 이용한 Nocardia sp. H17-1의 검출

Authors 

Kyung Hwa BaekYoung Ki LeeI S LeeHee-Mock OhByung Dae YoonHee Sik Kim

Publisher 

The Korean Society for Applied Microbiology and Biotechnology

Issue Date 

2004

Citation 

Korean Journal of Microbiology & Biotechnology, vol. 32, no. 1, pp. 91-95

Keywords 

denaturing gradient gel electrophoresis (DGGE)detectionl6S rDNAnocardia sp.PCRspecies-specific primerbacterium detectionbioremediationnocardiasoil pollution

Abstract 

For the detection of the oil-degrading bacterium, Nocardia sp. H17-1, inoculated during the bioremediation of oil-contaminated soil, a species-specific primer was constructed based on the 16S rDNA sequence of this strain. Two forward primers and two reverse primers were designed and tested against both closely and distantly related bacterial strains. All the primers designed were specific to the Nocardia sp. H17-1. Particularly, primer sets NH169F-NH972R and NH575F-NH972R could be used to detect 50 fg of template DNA and 1.2×104 CFU/g of sandy soil. These two PCR primer sets successfully detected the H17-1 strain in the oil-contaminated soil samples containing heterogeneous DNA. We also conformed the primer specificity by restriction-enzyme cleavage of the PCR products and denaturing gradient gel electrophoresis.

ISSN 

0257-2389

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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