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Title 

A method of high frequency virus-induced gene silencing in chili pepper(Capsicum annuum L. cv. Bukang)

Authors 

Eunsook ChungEun Soo SeongYeoung Cheol KimEun Joo ChungSang-Keun OhSanghyeob LeeJeong Mee ParkYoung Hee JoungDo Il Choi

Publisher 

Springer Verlag (Germany)

Issue Date 

2004

Citation 

Molecules and Cells, vol. 17, no. 2, pp. 377-380

Keywords 

C. annuumN. benthamianapeppersuppressionTRVVIGSoxygenasephytogene desaturaseribulosebisphosphate carboxylaseRNA directed RNA polymerase

Abstract 

Using a tobacco rattle virus (TRV)-based virus-induced gene silencing (VIGS) system, expression of phytogene desaturase (PDS) and ribulose-1,5-bisphosphate carboxylase small-subuit (rbcS) genes was suppressed in Nicotiana benthamiana and pepper plants (Capsicum annuum L. cv. Bukang). The silenced phenotypes of pale yellow (rbcS), and photobleached leaves (PDS), were invariably obvious 2 weeks after inoculation with the TRV-based vector. In a parallel experiment, the same set of genes was silenced in N. benthamiana and yielded identical phenotypes to pepper 1 week after inoculation. Northern blot analyses showed that the endogenous levels of CarbcS and CaPDS transcripts were dramatically reduced in the silenced leaf tissues. These observations confirm that the silenced phenotype is closely correlated with the pattern of tissue expression. To our knowledge, this is the first high frequency VIGS method in pepper plants. It should provide a tool for large-scale gene silencing studies in pepper functional genomics.

ISSN 

1016-8478

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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