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Title 

Development of PCR-ELISA for detection of HIV-1 in biomedical products

 

생물학적 제제에서 PCR-ELISA법을 이용한 HIV-1 오염 검색

Authors 

Jung Hyun ShimJ W KimKyung-Ae LeeDur Han KwonP K MyungJ O KimS H LeeS N ParkDo Young Yoon

Issue Date 

2004

Citation 

Korean Journal of Laboratory Medicine, vol. 24, no. 1, pp. 60-66

Keywords 

HIV-1PCR-ELISA

Abstract 

Background : Biomedical products such as viral vaccines can be contaminated with hazardous viruses during manufacturing processes and storage, thus causing harmful side effects. To assure the safety of biomedical products, highly effective and sensitive methods should be available to detect contaminating viruses. In this study, we performed recovery tests to determine the limit of detection of HIV-1. Methods : An HIV-1 plasmid preparation was serially diluted and spiked into various culture media (DMEM, RPMI-1640, IMDM, GICM, and SDM) containing 10% fetal bovine serum (FBS). The HIV-1 plasmid was detected by PCR alone or a combination of PCR and ELISA (PCR/ELISA). Results : When spiked into DMEM, RPMI, and IMDM, less than 4×10-2 ng of HIV-1 plasmid was not detectable as HIV-1 PCR products in agarose gel. Intra- and inter-assays (n=6) showed that the PCR-ELISA system could detect PCR products diluted as much as 1,875 times from HIV-1 plasmid serially spiked in various media. Conclusions : The PCR/ELISA system can be useful for the detection of trace amounts of hazardous viruses which may be present as contaminants in biological products.

ISSN 

1598-6535

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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