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Title 

Plant regeneration from immature zygotic embryo-derived embryogenic calluses and cell suspension cultures of Catharanthus roseus

Authors 

Suk Weon KimD S InP S ChoiJang Ryol Liu

Publisher 

Springer Verlag (Germany)

Issue Date 

2004

Citation 

Plant Cell Tissue and Organ Culture, vol. 76, no. 2, pp. 131-135

Keywords 

apocynaceaeimmature zygotic embryomadagascar periwinklesomatic embryosomatic embryogenesisplant cell culturecell suspensionsplant regenerationplants (botany)catharanthus roseus

Abstract 

Culture conditions for plant regeneration in immature zygotic embryo-derived embryogenic cell suspension cultures of Catharanthus roseus (Madagascar periwinkle) 'Little Bright Eye' are described. Immature zygotic embryos formed off-white, friable calluses at a frequency of 20% on Murashige and Skoog (MS) medium supplemented with 4.52 μM 2,4-dichlorophenoxyacetic acid (2,4-D) after 8 weeks of culture. After a second subculture using MS basal medium at 4-week intervals, off-white friable calluses formed a small quantity of yellowish, compact embryogenic calluses. Upon transfer to MS basal medium, embryogenic calluses gave rise to numerous somatic embryos. Cell suspension cultures were established with embryogenic calluses using liquid MS medium supplemented with 4.52 μM 2,4-D. Embryogenic cell clumps from cell suspension cultures developed into plantlets at a frequency of 56.7% when plated onto MS basal medium. Plantlets were transplanted to potting soil and grown to maturity in a growth chamber.

ISSN 

0167-6857

Link 

http://dx.doi.org/10.1023/B:TICU.0000007254.51387.7f

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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