상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Sequence-based approach to finding functional lipases from microbial genome databases

Authors 

H K KimYeo Jin JungWon Chan ChoiHyoung Seok RyuTae Kwang OhJung-Kee Lee

Publisher 

Wiley-Blackwell

Issue Date 

2004

Citation 

FEMS Microbiology Letters, vol. 235, no. 2, pp. 349-355

Keywords 

agrobacterium tumefaciensarchaeoglobus fulgidusdeinococcus radioduranslipasemicrobial genome databasetriacylglycerol lipaseamino acid sequencegenetic databasemicrobial geneticsnucleotide sequence

Abstract 

A sequence-based approach was used to retrieve functional lipases from microbial genome databases. Many novel genes assigned as putative lipases were tested using the criteria of the typical lipase sequence rule, based on a consensus sequence of a catalytic triad (Ser, Asp, His) and oxyanion hole sequence (HG). To obtain the lipase genes satisfying the sequence rule, PCR cloning was performed, while the lipase activities were tested using a tributyrin/tricaprylin plate and p-nitrophenyl caproate. Among nine putative lipases from four strains, five functional lipolytic proteins were obtained from Archaeoglobus fulgidus, Deinococcus radiodurans, and Agrobacterium tumefaciens. All five lipases exhibited a relatively low sequence similarity (less than 26.7%) with known lipases and turned out to belong to different lipase families. Accordingly, the current results indicate that the proposed strategic approach based on the microbial genome is an efficient and rapid method for finding novel and functional lipases.

ISSN 

0378-1097

Link 

http://dx.doi.org/10.1016/j.femsle.2004.05.004

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)