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Title 

Evaluation of cellulose-binding domain fused to a lipase for the lipase immobilization

Authors 

S P HwangJungoh AhnS M LeeT G LeeS J HaamK T LeeI S AhnJoon Ki Jung

Publisher 

Springer Verlag (Germany)

Issue Date 

2004

Citation 

Biotechnology Letters, vol. 26, no. 7, pp. 603-605

Keywords 

adsorptionavicelcellulose-binding domainimmobilizationlipasecellulasecellulosetriacylglycerol lipasevegetable oilenzyme stability

Abstract 

A cellulose-binding domain (CBD) fragment of a cellulase gene of Trichoderma hazianum was fused to a lipase gene of Bacillus stearothermophilus L1 to make a gene cluster for CBD-BSL lipase. The specific activity of CBD-BSL lipase for oil hydrolysis increased by 33% after being immobilized on Avicel (microcrystalline cellulose), whereas those of CBD-BSL lipase and BSL lipase decreased by 16% and 54%, respectively, after being immobilized on silica gel. Although the loss of activity of an enzyme immobilized by adsorption has been reported previously, the loss of activity of the CBD-BSL lipase immobilized on Avicel was less than 3% after 12 h due to the irreversible binding of CBD to Avicel.

ISSN 

0141-5492

Link 

http://dx.doi.org/10.1023/B:BILE.0000021964.69500.6f

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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