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Title 

Phylogenetic analysis of the genera Streptomyces and Kitasatospora based on partial RNA polymerase β-subunit gene (rpoB) sequences

Authors 

B J KimChang-Jin KimJ S ChunY H KohS H LeeJ W HyunC Y ChaY H Kook

Publisher 

Society for General Microbiology

Issue Date 

2004

Citation 

International Journal of Systematic and Evolutionary Microbiology, vol. 54, no. 2, pp. 593-598

Keywords 

RNA polymerasegene sequencegenetic analysisKitasatosporanucleotide sequencepolymerase chain reactionpriority journalsequence homologyDNA-Directed RNA Polymerasesstreptomyces

Abstract 

The RNA polymerase β-subunit genes (rpoB) of 67 Streptomyces strains, representing 57 species, five Kitasatospora strains and Micromonospora echinospora KCTC 9549 were partially sequenced using a pair of rpoB PCR primers. Among the streptomycetes, 99.7-100% similarity within the same species and 90.2-99.3% similarity at the interspecific level were observed by analysis of the determined rpoB sequences. The topology of the phylogenetic tree based on rpoB sequences was similar to that of 16S rDNA. The five Kitasatospora strains formed a stable monophyletic clade and a sister group to the clade comprising all Streptomyces species. Although there were several discrepancies in the details, considerable agreement was found between the results of rpoB analysis and those of numerical phenetic classification. This study demonstrates that analysis of rpoB can be used as an alternative genetic method in parallel to conventional taxonomic methods, including numerical phenetic and 16S rDNA analyses, for the phylogenetic analyses of the genera Streptomyces and Kitasatospora.

ISSN 

1466-5026

Link 

http://dx.doi.org/10.1099/ijs.0.02941-0

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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