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Title 

Plant regeneration from hairy-root cultures transformed by infection with Agrobacterium rhizogenes in Catharanthus roseus

Authors 

P S ChoiY D KimK M ChoiH J ChungD W ChoiJang Ryol Liu

Publisher 

Springer Verlag (Germany)

Issue Date 

2004

Citation 

Plant Cell Reports, vol. 22, no. 11, pp. 828-831

Keywords 

agrobacterium rhizogenesapocynaceaemadagascar periwinkletransgenic plantplants (botany)infectionprolific rootingplant cell culturecatharanthusculture medium

Abstract 

Hypocotyl explants of Catharanthus roseus produced hairy roots when cultured on Murashige and Skoog (MS) basal medium after infection by Agrobacterium rhizogenes. Explants gave rise to adventitious shoots at a frequency of up to 80% when cultured on MS medium supplemented with 31.1 μM 6-benzyladenine and 5.4 μM α-naphthaleneacetic acid. There was a significant difference in the frequency of adventitious shoot formation for each hairy-root line derived from a different cultivar. Plants derived from hairy roots exhibited prolific rooting and had shortened internodes. Approximately half of the plants had wrinkled leaves and an abundant root mass with extensive lateral branching, but otherwise appeared morphologically normal. Plants with hairy roots that were derived from the cultivar Cooler Apricot developed flowers with petals that were white in the proximal region, whereas the wild-type flower petals are red. PCR and Southern blot analyses revealed that plants derived from hairy roots retained the Ri TL-DNA.

ISSN 

0721-7714

Link 

http://dx.doi.org/10.1007/s00299-004-0765-3

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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