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Title 

Up-regulation of Bfl-1/A1 via NF-κB activation in cisplatin-resistant human bladder cancer cell line

Authors 

Jin Koo KimKwang Dong KimE S LeeJong-Seok LimHee Jun ChoHyun Kyung YoonMi Young ChoKyoung Eun BaekYuk-Pheel ParkS G PaikYong Kyung ChoeHee Gu Lee

Publisher 

Elsevier

Issue Date 

2004

Citation 

Cancer Letters, vol. 212, no. 1, pp. 61-70

Keywords 

Bfl-1, Bcl-2-related gene expressed in fetal liverBfl-1/A1cisplatin, cis-diamminedichloroplatinum (II)cisplatin-resistant bladder cancerdifferential displayNF-κBcisplatinimmunoglobulin enhancer binding proteinprotein bcl 2bladder carcinoma

Abstract 

The potent anti-cancer agent cis-diamminedichloroplatinum (II) (cisplatin) is currently used for treating bladder cancer. However, clinical use of this drug for long periods is often limited because of the appearance of cisplatin-resistant bladder tumor cells. We employed the method of a differential display reverse transcriptase polymerase chain reaction to identify the differentially expressed genes in the parental human bladder cancer cell line, T24 and three cisplatin-resistant cell lines. We report here that cisplatin-resistant cell lines overexpress Bcl-2 family protein Bcl-2-related gene expressed in fetal liver (Bfl-1)/A1 as compared with their parental cell. Cisplatin and γ-irradiation induced expression of Bfl-1/A1 in T24R2 cells but not in T24 cells. Among Bcl-2 family members, Bfl-1/A1 showed the most significant alteration of the expression level in resistant cells. The nuclear translocation of nuclear factor-kappaB (NF-κB) by cisplatin and γ-irradiation selectively occurred in T24R2 cells. Mitochondrial depolarization and cell death by cisplatin were also prevented in T24R2 cells. Moreover, Bfl-1/A1 inhibited cisplatin- and TNF-α-induced apoptosis in BOSC23 cells. Our findings suggest that the induction of Bfl-1/A1 by NF-κB may be important in controlling resistance to cisplatin responses in bladder tumor cells.

ISSN 

0304-3835

Link 

http://dx.doi.org/10.1016/j.canlet.2004.02.021

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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