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Title 

EST and microarray analyses of pathogen-responsive genes in hot pepper ( Capsicum annuum L.) non-host resistance against soybean pustule pathogen ( Xanthomonas axonopodis pv. glycines)

Authors 

Sanghyeob LeeSoo Yong KimEun Joo ChungYoung Hee JoungH S PaiCheol-Goo HurDoil Choi

Issue Date 

2004

Citation 

Functional & Intergrative Genomics, vol. 4, no. 3, pp. 196-205

Keywords 

capsicum annuumESTnon-host resistancetranscriptome analysisgibberellincarbohydrate metabolismcell infiltrationcell transportchloroplast geneticscold

Abstract 

Large-scale single-pass sequencing of cDNA libraries and microarray analysis have proven to be useful tools for discovering new genes and studying gene expression. As a first step in elucidating the defense mechanisms in hot pepper plants, a total of 8,525 expressed sequence tags (ESTs) were generated and analyzed in silico. The cDNA microarray analysis identified 613 hot pepper genes that were transcriptionally responsive to the non-host soybean pustule pathogen Xanthomonas axonopodis pv. glycines (Xag). Several functional types of genes, including those involved in cell wall modification/biosynthesis, transport, signaling pathways and divergent defense reactions, were induced at the early stage of Xag infiltration. In contrast, genes encoding proteins that are involved in photosynthesis, carbohydrate metabolism and the synthesis of chloroplast biogenetic proteins were down-regulated at the late stage of Xag infiltration. These expression profiles share common features with the expression profiles elicited by other stresses, such as fungal challenge, wounding, cold, drought and high salinity. However, we also identified several novel transcription factors that may be specifically involved in the defense reaction of the hot pepper. We also found that the defense reaction of the hot pepper may involve the deactivation of gibberellin. Furthermore, many genes encoding proteins with unknown function were identified. Functional analysis of these genes may broaden our understanding of non-host resistance. This study is the first report of large-scale sequencing and non-host defense transcriptome analysis of the hot pepper plant species. (The sequence data in this paper have been submitted to the dbEST and GenBank database under the codes 10227604-10236595 and BM059564-BM068555, respectively. Additional information is available at http://plant.pdrc.re.kr/ks200201/pepper.html.

ISSN 

1438-793X

Link 

http://dx.doi.org/10.1007/s10142-003-0099-1

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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