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Title 

High production of L-threonine using controlled feeding of L-methionine and phosphate by Escherichia coli mutant

 

L-methionine과 phosphate의 제한 공급에 의한 Escherichia coli MT201로부터의 고농도 L-threonine 생산

Authors 

Man Hyo LeeHong-Weon LeeB J KimChun Suk KimJoon Ki JungY I Hwang

Publisher 

The Korean Society for Applied Microbiology and Biotechnology

Issue Date 

2004

Citation 

Korean Journal of Microbiology & Biotechnology, vol. 32, no. 2, pp. 149-153

Keywords 

escherichia coli MT201L-MethionineL-Threonine productionphosphateyeast extractmethioninethreoninebacterial growthbacterium culture

Abstract 

L-Threonine fermentation process was constructed on batch and fed-batch culture by using Escherichia coli MT201. The production type of L-threonine was observed as growth-associated production in batch culture. In fed-batch culture studying optimal concentration of yeast extract in feeding media, when 600 g/l of glucose and 60 g/l of yeast extract were added in feeding media, 87 g/l of L-threonine was produced. To improve cell growth and L-threonine production, the culture of high cell density was performed in fed-batch culture with oxygen enriched air and feeding media containing L-methionine and phosphate. Under the conditions, we could achieve the highest L-threonine production of 98 g/l at 60 h. The highest productivity of L-threonine was about 3.85 g/l/h.

ISSN 

0257-2389

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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