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Title 

Impeller types and feeding modes influence the morphology and protein expression in the submerged culture of Aspergillus oryzae

Authors 

Joo-Hyung HeoV AnaninJeong Seok ParkChung Ryul LeeJ O MoonOh Suk KwonHyun Ah KangChul Ho KimSang Ki Rhee

Publisher 

Springer Verlag (Germany)

Issue Date 

2004

Citation 

Biotechnology and Bioprocess Engineering, vol. 9, no. 3, pp. 184-190

Keywords 

aspergillus oryzaefed-batch fermentationfilamentous fungimorphologypropeller agitationsubmerged culture

Abstract 

The influences of impeller types on morphology and protein expression were investigated in a submerged culture of Aspergillus oryzae. The impeller types strongly affected mycelial morphology and protein production in batch and fed-batch fermentations. Cells that were cultured by propeller agitation grew in the form of a pellet, whereas cells that were cultured by turbine agitation grew in a freely dispersed-hyphal manner and in a clumped form. Pellet-grown cells showed high levels of protein production for both the intracellularly heterologous protein (β-glucuronidase) and the extracellularly homologous protein (α-amylase). The feeding mode of the carbon source also influenced the morphological distribution and protein expression in fed-batch fermentation of A. oryzae. Pulsed-feeding mainly showed high protein expression and homogeneous distribution of pellet whereas continuous feeding resulted in less protein expression and heterogeneous distribution with pellet and dispersed-hyphae. The pellet growth with propeller agitation paralleling with the pulsed-feeding of carbon source showed a high level of protein production in the submerged fed-batch fermentation of recombinant A. oryzae.

ISSN 

1226-8372

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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