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Title 

Adeno-associated virus Rep78 binds to E2-responsive element 1 of bovine papillomavirus type 1

Authors 

S K ChonB M RimDong Soo Im

Publisher 

Taylor & Francis

Issue Date 

1999

Citation 

IUBMB Life, vol. 48, no. 4, pp. 397-404

Abstract 

Adeno-associated virus type-2 (AAV-2) is a helper-dependent parvovirus that has been implicated in the inhibition of replication and oncogenic transformation of bovine papillomavirus type-1 (BPV-1) and other transforming DNA viruses. Previous studies have suggested that the Rep78 protein of AAV-2 is a key player mediating this effect. In this report we have analyzed the effect of AAV-2 Rep78 protein on the regulation of gene expression of a reporter gene under the control of the long control region (LCR) of BPV-1. Our results show that Rep78 is capable of down-regulating the promoter activity of the LCR in vivo in tissue culture cells. Inhibition of LCR activity in vivo suggested the need for Rep78 to bind to a region of the LCR promoter spanning the E2-responsive elements of BPV-1. This observation was further confirmed in vitro with gel shift assays showing specific binding of Rep78 to DNA oligonucleotides containing E2-responsive element 1 (E2RE1) sequences of BPV-1 LCR. Our results expand the understanding of the mechanism of trans-regulation mediated by Rep78 and involving this protein and DNA sequences with complex secondary structure.

ISSN 

1521-6543

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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