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Title 

Analysis of microbial communities during cyanobacterial bloom in Daechung reservoir by DGGE

 

DGGE를 이용한 대청호 수화 발생시기의 세균군집 분석

Authors 

So-Ra KoS J ParkChi-Yong AhnAe-Ran ChoiJung-Sook LeeHee Sik KimByung Dae YoonHee-Mock Oh

Publisher 

The Microbiological Society of Korea

Issue Date 

2004

Citation 

Korean Journal of Microbiology, vol. 40, no. 3, pp. 205-210

Keywords 

algal bloomcyanobacteriaDGGE (denaturing gradient gel electrophoresis)16S rDNA

Abstract 

The change of bacterial communities during cyanobacterial bloom was analyzed by DGGE in Daechung Reservoir from July to October in 2003. The traditional morphological analysis showed that the genera of Microcystis, Chroococcus, Oscillatoria, and Phormidium were dominated. The most frequent band in the DGGE profile by 16S rDNA sequence analysis was identified as Microcystis flos-aquae and the cyanobacterial bloom was peaked on September 2. Oscillatoria spp. were also identified and Aphanizomenon flos-aquae dominated in the middle of August. Judging from the analysis of the digitalized DGGE profiles using the cluster analysis technique, the microbial community on September 2 was considerably different from others. Consequently, it seems that the gene fingerprinting method can give not only the similar results to the traditional morphological method but also additional information on the bacterial species and similarity among the examined microbial communities.

ISSN 

0440-2413

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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