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Title 

DNA gyrase is involved in chloroplast nucleoid partitioning

Authors 

Hye Sun ChoSang Sook LeeKwang Dong KimI H HwangJong-Seok LimY I ParkH S Pai

Publisher 

American Society of Plant Biologists

Issue Date 

2004

Citation 

Plant Cell, vol. 16, no. 10, pp. 2665-2682

Keywords 

DNA gyraseDNADNA topoisomerase (ATP hydrolysing)primer DNAchloroplastDNA primers

Abstract 

DNA gyrase, which catalyzes topological transformation of DNA, plays an essential role in replication and transcription in prokaryotes. Virus-induced gene silencing of NbGyrA or NbGyrB, which putatively encode DNA gyrase subunits A and B, respectively, resulted in leaf yellowing phenotypes in Nicotiana benthamiana. NbGyrA and NbGyrB complemented the gyrA and gyrB temperature-sensitive mutations of Escherichia coli, respectively, which indicates that the plant and bacterial subunits are functionally similar. NbGyrA and NbGyrB were targeted to both chloroplasts and mitochondria, and depletion of these subunits affected both organelles by reducing chloroplast numbers and inducing morphological and physiological abnormalities in both organelles. Flow cytometry analysis revealed that the average DNA content in the affected chloroplasts and mitochondria was significantly higher than in the control organelles. Furthermore, 4′,6-diamidino-2-phenylindole staining revealed that the abnormal chloroplasts contained one or a few large nucleoids instead of multiple small nucleoids dispersed throughout the stroma. Pulse-field gel electrophoresis analyses of chloroplasts demonstrated that the sizes and/ or structure of the DNA molecules in the abnormal chloroplast nucleoids are highly aberrant. Based on these results, we propose that DNA gyrase plays a critical role in chloroplast nucleoid partitioning by regulating DNA topology.

ISSN 

1040-4651

Link 

http://dx.doi.org/10.1105/tpc.104.024281

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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