상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Secretion and localization of Pseudomonas aurantiaca levansucrase expressed in Saccharomyces cerevisiae

 

Saccharomyces cerevisiae에서 발현된 Pseudomonas aurantiaca levansucrase의 분비국재성

Authors 

C K LimK H KimChul Ho KimSang Ki RheeS W Nam

Publisher 

The Korean Society for Applied Microbiology and Biotechnology

Issue Date 

2004

Citation 

Korean Journal of Microbiology & Biotechnology, vol. 32, no. 3, pp. 206-211

Keywords 

exoinulinase signallevansucrasepseudomonas aurantiacasacchromayces cerevisiaeenzyme localizationpseudomonas

Abstract 

Levansucrase gene(lscA) from Pseudomonas aurantiaca was subcloned downstream of GAL1 promoter in pYES 2.0 and pYInu-AT [GAL10 promoter + exoinulinase signal sequence of Kluyveromyces marxianus], resulting pYES-lscA and pYInu-lscA, respectively. The two expression plasmids were introduced into an invertase-deficient strain, Sacchromayces cerevisiae SEY2102, and transformants with high activity of levansucrase were selected. When each yeast transformants was cultivated in medium containing galactose, the extracellular and intracellular activities of levansucrase reached about 8.62 U/ml with the strain harboring pYES-lscA and 5.43 U/ml with the strain harboring pYInu-lscA. The levansucrase activity of 80% was detected in the periplasmic space and cytoplasm. The levansucrase activity in the medium of SEY2102/pYInu-lscA was 0.87 U/ml whereas that of SEY2102/pYES-lscA was 0.47 U/ml, which implying the exoinulinase signal sequence didn't enhance the secretion efficiency of levansucrase. Furthermore, the recombinant levansucrase expressed in yeast seems to be produced as a hyper-glycosylated form.

ISSN 

0257-2389

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)