상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Effective production of microinjectable blastocysts for Germ-line transmission of embryonic stem cells

Authors 

Sun-Uk KimYing Hao HanT H LeeByung Hwa HyunS H LeeDong Seok LeeDae Yeul Yu

Publisher 

Japanese Association for Laboratory Animal Sciences

Issue Date 

2004

Citation 

Experimental Animals, vol. 53, no. 5, pp. 475-477

Keywords 

C57BL/6 micegerm-line chimerahost embryoanimal cellanimal cell cultureblastocystembryo cellgerm linestem cellcell culture

Abstract 

Blastocysts of C57BL/6 mice obtained either 2.5 or 3.5 days post-coitum (dpc) were examined for efficient microinjection after overnight in vitro culture. Incidences of zona-free embryos were much higher at 3.5 dpc after natural mating (1.05/mouse) and superovulation (2.83/mouse) than at 2.5 dpc after natural mating (0.05/mouse) and superovulation (0.01/mouse). By testing germ-line competency of gene-targeted J1 embryonic stem cells, superovulation and/or in vitro culture should be recommended for producing microinjectable blastocysts for production of high germ-line chimeras.

ISSN 

1341-1357

Link 

http://dx.doi.org/10.1538/expanim.53.475

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)