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Title 

Polo-box motif targets a centrosome regulator, RanGTPase

Authors 

Young Joo JangJae-Hoon JiJi Hee AhnKwang Lae HoeMi Sun WonDong Soo ImS K ChaeS SongHyang Sook Yoo

Publisher 

Elsevier

Issue Date 

2004

Citation 

Biochemical and Biophysical Research Communications, vol. 325, no. 1, pp. 257-264

Keywords 

centrosomepolo-boxpolo-like kinaseRanBPMRanGTPaseguanosine triphosphatasenocodazolephosphotransferasepolo like kinaseRan protein

Abstract 

Mammalian polo-like kinase (Plk) acts at various stages in early and late mitosis. Plk1 localizes in the centrosome, the central spindle, the midbody as well as the kinetochore. The non-catalytic region in the C-terminus of Plk1 has conserved sequence motifs, named polo-boxes. These motifs are important for Plk localization. GFP protein fused with the core sequences of polo-box (50 amino acids) localized Plk to target organelles. We screened for Plk interacting proteins by constructing a tandem repeat of the polo-box motif, and used it as bait in the two-hybrid system with HeLa cell cDNA library. RanGTPase was detected as a positive clone. Through in vitro and in vivo protein binding analysis in synchronized cells by thymidine block and by nocodazole treatment, we confirmed the interaction between endogenous Ran and Plk1. We showed that endogenous Ran and Plk1 proteins were co-localized to centrosomes, which is a major target organelle of endogenous Plk1, in early mitotic cells by immunofluorescence. Finally, we demonstrated that Plk1 phosphorylated RanBPM, a Ran-binding protein in microtubule organizing center, through the interaction with Ran. These data suggested that the core motif of polo-box is sufficient for Plk1-targeting, and that Plk1 may play roles in centrosome through recruitment and/or activation of Ran/RanBPM proteins.

ISSN 

0006-291X

Link 

http://dx.doi.org/10.1016/j.bbrc.2004.10.023

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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