상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

CpG methylation of the mouse CYP1A2 promoter

Authors 

B JinD W ParkKi Hoan NamG T OhY S LeeD Y Ryu

Publisher 

Elsevier

Issue Date 

2004

Citation 

Toxicology Letters, vol. 152, no. 1, pp. 11-18

Keywords 

5-aza-2′-deoxycytidineAhRARNTarylhydrocarbon receptorarylhydrocarbon receptor nuclear translocatorAzaCbasic helix-loop-helixBhlhCYPcytochrome P450

Abstract 

Cytochrome P450 1A2 (CYP1A2) is a xenobiotic metabolizing enzyme that is tissue-specifically regulated in the mammalian liver by arylhydrocarbon receptor (AhR)-dependent and -independent pathways. In this study, CpG methylation of the CYP1A2 promoter was analyzed in mouse tissues and liver-derived cells. Compared to lung and kidney, the CYP1A2 promoter is undermethylated in the liver in a promoter domain-specific manner. The CYP1A2 promoter showed a similar methylation pattern in wild-type and AhR-null liver. At birth, the promoter was hypermethylated and CYP1A2 was negligibly expressed in the liver. However, CYP1A2 expression increased following birth, coincident with the demethylation of the promoter. In hepatoma Hepa1c1c7 cells not expressing CYP1A2, the promoter was hypermethylated at specific CpG sites. In isolated hepatocytes, CYP1A2 expression declined over time and the degree of CYP1A2 methylation increased, albeit only after a delay. Exposure to 5-aza-2′-deoxycytidine did not induce CYP1A2 in Hepa1c1c7 cells and hepatocytes. Taken together, our findings suggest that CpG methylation is involved in the tissue-specific and developmental regulation of CYP1A2, but the de novo methylation of the CYP1A2 promoter is induced by the silent state of the gene rather than causing it.

ISSN 

0378-4274

Link 

http://dx.doi.org/10.1016/j.toxlet.2004.03.016

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)