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Title 

Immunization with virus-like particle of human papillomavirus type 16 L1 elicits CTL immune response in C57BL/6 mice

Authors 

Hyun Mi PyoJ Y ParkS N ParkH S KimKee Sun ShinHaryoung Poo

Publisher 

Trans Tech Publications

Issue Date 

2005

Citation 

Key Engineering Materials, vol. 277, no. 0, pp. 119-124

Keywords 

chromium release assaycytotoxic T lymphocyte (CTL)human papillomavirus (HPV)lymphoproliferation assayvirus-like particle (VLP)viruseshuman papillomavirusvirus-like particleimmunization

Abstract 

The development of a human papillomavirus vaccine has received a lot of recent attention due to the connection between HPV infections and cervical cancer. One promising vaccine to prevent HPV infections is an HPV virus-like particle, and various studies have already shown that HPV VLP immunization elicits a humoral immune response. However, cell mediated immunity is important for the prevention of HPV infections and cancer therapy. Therefore, to check the elicitation of a CTL response by yeast-derived virus- like particles consisting of the HPV 16 capsid protein L1, HPV 16 L1 VLPs were produced and purified, then C57BL/6 mice immunized with the HPV 16 L1 VLPs by subcutaneous injection. Thereafter, the splenocytes from the immunized mice were isolated and a chromium release assay performed using recombinant B16/HPV16 L1cells constantly expressing the HPV16 L1 protein. As a result, the data demonstrated that the T cells from the HPV16 L1 VLP immunized mice exhibited a significantly higher cytotoxic T lymphocyte activity against the recombinant B 16/HPV 16 L1 cells than the T cells from the PBS immunized control mice.

ISSN 

1013-9826

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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