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Title 

Selection of high ginsenoside producing ginseng hairy root lines using targeted metabolic analysis

Authors 

S S WooJ S SongJ Y LeeD S InHwa-Jee ChungJang Ryol LiuD W Choi

Publisher 

Elsevier

Issue Date 

2004

Citation 

Phytochemistry, vol. 65, no. 20, pp. 2751-2761

Keywords 

araliaceae, hairy root ginsenoside biosynthesismetabolic profilingpanax ginsengginsenosidechemical analysiscluster analysisginsenghigh performance liquid chromatographyplant rootqualitative analysis

Abstract 

Ginseng hairy root lines which varied significantly in ginsenoside production were selected by principle component analysis and ginsenoside profiling using LC/MS and HPLC-UV analyses. To develop an experimental system for studying ginsenoside biosynthesis, we generated thousands of ginseng (Panax ginseng C.A. Meyer) hairy roots, genetically transformed roots induced by Agrobacterium rhizogenes, and analyzed the ginsenosides in the samples. 27 putative ginsenosides were detected in ginseng hairy roots. Quantitative and qualitative variations in the seven major ginsenosides were profiled in 993 ginseng hairy root lines using LC/MS and HPLC-UV. Cluster analysis of metabolic profiling data enabled us to select hairy root lines, which varied significantly in ginsenoside production. We selected hairy root lines producing total ginsenoside contents 4-5 times higher than that of a common hairy root population, as well as lines that varied in the ratio of the protopanaxadiol to protopanaxatriol type ginsenoside. Some of the hairy root lines produce only a single ginsenoside in relatively high amounts. These metabolites represent the end product of gene expression, thus metabolic profiling can give a broad view of the biochemical status or biochemical phenotype of a hairy root line that can be directly linked to gene function.

ISSN 

0031-9422

Link 

http://dx.doi.org/10.1016/j.phytochem.2004.08.039

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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