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Title 

Drosophila short neuropeptide F regulates food intake and body size

Authors 

Kyu-Sun LeeK H YouJ G ChooYong Mahn HanKweon Yu

Publisher 

American Society for Biochemistry and Molecular Biology

Issue Date 

2004

Citation 

Journal of Biological Chemistry, vol. 279, no. 49, pp. 50781-50789

Keywords 

food productsfeeding behaviorsneuropeptidesdrosophila proteinneuropeptide funclassified drugbody sizedrosophila melanogasterfood intakegene function

Abstract 

Neuropeptides regulate a wide range of animal behavior including food consumption, circadian rhythms, and anxiety. Recently, Drosophila neuropeptide F, which is the homolog of the vertebrate neuropeptide Y, was cloned, and the function of Drosophila neuropeptide F in feeding behaviors was well characterized. However, the function of the structurally related short neuropeptide F (sNPF) was unknown. Here, we report the cloning, RNA, and peptide localizations, and functional characterizations of the Drosophila sNPF gene. The sNPF gene encodes the preprotein containing putative RLRF amide peptides and was expressed in the nervous system of late stage embryos and larvae. The embryonic and larval localization of the sNPF peptide in the nervous systems revealed the larval central nervous system neural circuit from the neurons in the brain to thoracic axons and to connective axons in the ventral ganglion. In the adult brain, the sNPF peptide was localized in the medulla and the mushroom body. However, the sNPF peptide was not detected in the gut. The sNPF mRNA and the peptide were expressed during all developmental stages from embryo to adult. From the feeding assay, the gain-of-function sNPF mutants expressed in nervous systems promoted food intake, whereas the loss-of-function mutants suppressed food intake. Also, sNPF overexpression in nervous systems produced bigger and heavier flies. These findings, indicate that the sNPF is expressed in the nervous systems to control food intake and regulate body size in Drosophila melanogaster.

ISSN 

0021-9258

Link 

http://dx.doi.org/10.1074/jbc.M407842200

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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