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Title 

IL-18 enhances SCF production of melanoma cells by regulating ROI and p38 MAPK activity

Authors 

J HueA KimH SongIn Pyo ChoiH ParkT KimW J LeeH KangD Cho

Publisher 

Elsevier

Issue Date 

2005

Citation 

Immunology Letters, vol. 96, no. 2, pp. 211-217

Keywords 

IL-18melanomap38 MAPKROISCFinterleukin 18mitogen activated protein kinase p38stem cell factoranimal cellcell proliferation

Abstract 

It has been reported that interleukin-18 (IL-18) is secreted by B16 murine melanoma cells and that this endogenous IL-18 is involved in the immune escape of murine melanoma cells. The present study investigated whether interleukin (IL)-18 can regulate stem cell factor (SCF) expression, known to be associated with melanocyte proliferation, in B16F10 murine melanoma cells. SCF expression was examined by RT-PCR, intracellular FACS analysis, and ELISA in IL-18 antisense transfectants. Transfection with IL-18 antisense cDNA reduced SCF expression and the expression was enhanced by addition of exogenous IL-18. In addition, the effect of IL-18 was blocked by the antioxidant, N-acetyl-l-cysteine (NAC), indicating that IL-18 regulates ROI production, which is involved in SCF production. Furthermore, inhibitors of p38 mitogen-activated protein kinase (MAPK), such as SB203580, blocked enhanced SCF expression, indicating that p38 MAPK activity is required for IL-18-enhanced SCF production. Taken together, these results suggest that IL-18 plays a critical role as a regulatory factor of SCF expression via ROI and p38 MAPK activity in B16F10 murine melanoma cells.

ISSN 

0165-2478

Link 

http://dx.doi.org/10.1016/j.imlet.2004.08.008

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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