상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Effects of 3,5-di-O-Caffeoylquinic acid from Artemisia scoparia waldstein et kitamura on the function of HPV 16 oncoproteins

 

인진에서 분리한 3,5-di-O-Caffeoylquinic acid가 자궁경부암 바이러스 발암단백질의 기능에 미치는 영향

Authors 

Tae Woong BaekKyung Ae LeeMin Jung AhnHae Kyung JooMin Chul ChoJeong Woo KangHee Seo KimJung Hyun ShimHee Gu LeeHyuncheol OhJong Seog AhnY K ChoP K MyungDo Young Yoon

Publisher 

Korean Society of Pharmacognosy

Issue Date 

2004

Citation 

Korean Journal of Pharmacognosy, vol. 35, no. 4, pp. 368-374

Keywords 

3,5-di-o-caffeoylquinic acidartemisia scoparia waldstein et kitamuraE6E6APhuman papilloma virus (HPV)3,5 di o caffeoylquinic acidantineoplastic agentnatural productoncoproteinoncoprotein inhibitor

Abstract 

Cervical cancer is one of the leading causes of female death. Viral oncoproteins E6 and E7 are selectively retained and expressed in carcinoma cells infected with HPV (Human papillomavirus) type 16. The HPV is cooperated in immotalization and transformation of primary keratinocyte. E6 and E7 oncoproteins interfere the functions of tumor suppressor proteins p53 and retinoblasoma protein (pRb), respectively. Among a lots of natural products, Artemisia scoparia Waldstein et Kitamura has inhibitory effects on the binding between E6 oncoprotein and tumor suppressor p53, or the binding between E6 and E6 associated protein (E6AP), an E3 ubiquitin-protein ligase. HPV oncoprotein inhibitors from Artemisia scoparia W. were isolated by solvent partition and column chromatography (Silica gel, RP-18) and the inhibitory compounds were finally purified by HPLC using an ELISA screening system based on the binding between E6 and E6AP. The aim of this study is to identify the structure of inhibitory compounds and to investigate whether these compounds have inhibitory effects on the functions of E6 oncoprotein. We investigated whether 3,5-di-O-caffeoylquinic acid (DCQA) extracted from Artemisia scoparia W. could inhibit the function of E6 oncoprotein. DCQA inhibited the in vitro binding of E6 and E6AP which are essential for the binding and degradation of the tumor suppressor p53 and also inhibited the proliferation of human cervical cancer cell lines (SiHa and CaSKi) in a dose response manner. These results suggest that DCQA inhibited the function of E6 oncoprotein, suggesting that it can be used as a potential drug for the treatment of cervical cancers infected with HPV.

ISSN 

0253-3073

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)