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Title 

Surface plasmon resonance imaging-based protein array chip system for monitoring a hexahistidine-tagged protein during expression and purification

Authors 

H S RoSun Ok JungByung Hoon KhoHyung Pyo HongJae Sung LeeYong Beom ShinMin-Gon KimBong Hyun Chung

Publisher 

American Society for Microbiology

Issue Date 

2005

Citation 

Applied and Environmental Microbiology, vol. 71, no. 2, pp. 1089-1092

Keywords 

acetic acidchemical analysischromatographyproteinssurface plasmon resonancechip systemcrude extractsrecombinant proteinimaging techniquescell extract

Abstract 

A surface plasmon resonance imaging-based Ni2+-iminodiacetic acid-coated gold chip system was developed to enable specific detection of a hexahistidine-tagged recombinant protein in crude extracts or in column chromatography fractions. This system is especially advantageous for high-throughput analysis of multiple proteins.

ISSN 

0099-2240

Link 

http://dx.doi.org/10.1128/AEM.71.2.1089-1092.2005

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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