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Title 

Somatic embryogenesis and plant regeneration in leaf and petiole explant cultures and cell suspension cultures of Pinellia tripartita

Authors 

Suk Weon KimD S InK H TaeJang Ryol Liu

Publisher 

Springer Verlag (Germany)

Issue Date 

2005

Citation 

Plant Cell Tissue and Organ Culture, vol. 80, no. 3, pp. 267-270

Keywords 

araceaeembryogenic culturesomatic embryoplant cell culturecell suspension culturesplantletsplants (botany)explantsleavesplants

Abstract 

Culture conditions for high frequency plant regeneration via somatic embryogenesis and from cell suspension cultures of Pinellia tripartita are described. Leaf and petiole explants formed embryogenic cultures at frequencies of 36.8% and 26.7%, respectively, on Murashige and Skoog (MS) medium supplemented with 1.33 μM 6-benzyladenine (BA) and 10.74 μM α-naphthaleneacetic acid (NAA). However, leaf and petiole explants did not form embryogenic cultures on MS medium with various concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) as the sole growth regulator. Leaf-derived embryogenic cultures were transferred to MS medium with 4.52 μM 2,4-D and were subcultured at 4-week intervals. Cell suspensions were established from subcultured embryogenic cultures using liquid MS medium with 4.52 μM 2,4-D. Upon plating onto MS basal medium, ≥70% of cell aggregates gave rise to somatic embryos and later plantlets. Regenerated plantlets were transplanted to potting soil and developed to maturity at a frequency of ≥90% in a growth chamber.

ISSN 

0167-6857

Link 

http://dx.doi.org/10.1007/s11240-004-1018-4

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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