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Title 

Molecular cloning of levan fructotransferase gene from Arthrobacter ureafaciens K2032 and its expression in Escherichia coli for the production of difructose dianhydride IV

Authors 

Chul Ho KimE K JangS H KimK H JangS A KangK B SongOh Suk KwonSang Ki Rhee

Publisher 

Wiley-Blackwell

Issue Date 

2005

Citation 

Letters in Applied Microbiology, vol. 40, no. 3, pp. 228-234

Keywords 

absorption factor of mineralsDFAfructosyltransferaselow-calorie sweetenerdifructose dianhydride ivlevanlevan fructotransferasearthrobacterarthrobacter ureafaciens k2032escherichia coli

Abstract 

Aims: To clone and overexpress a novel levan fructotransferase gene lftA from Arthrobacter ureafaciens K2032. Methods and Results: The lftA gene, encoding a levan fructotransferase (LFTase) of 521 amino acids (aa) residues, was cloned from the genomic DNA of A. ureafaciens K2032, and overexpressed in Escherichia coli. The recombinant LFTase overexpressed in E. coli was then used to produce a difructose dianhydride (DFA IV) from levan. DFA IV crystals with 97% purity could be obtained from the reaction mixture in 83-7% yield by using a natural crystallization method. Conclusions: The lftA gene cloned from A. ureafaciens K2032 encode a novel levan fructotransferase which produces difructose dianhydride (DFA IV) from levan. Significance and Impact of the Study: Levan fructotransferase is a useful enzyme with great promise in the production of DFA IV and various fructosides.

ISSN 

0266-8254

Link 

http://dx.doi.org/10.1111/j.1472-765X.2005.01658.x

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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