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Title 

Analyses of enzyme II gene mutants for sugar transport and heterologous expression of fructokinase gene in Corynebacterium glutamicum ATCC 13032

Authors 

Min Woo MoonHyo Jin KimTae Kwang OhC S ShinJ S LeeS J KimJung-Kee Lee

Publisher 

Wiley-Blackwell

Issue Date 

2005

Citation 

FEMS Microbiology Letters, vol. 244, no. 2, pp. 259-266

Keywords 

corynebacterium glutamicumenzyme IIfructokinasephosphotransferase systemsugar transportdeletion mutantgene deletiongene expressiongene functiongene mutation

Abstract 

Corynebacterium glutamicum ATCC 13032 has four enzyme II (EII) genes of the phosphotransferase system in its genome encoding transporters for sucrose, glucose, fructose, and an unidentified EII. To analyze the function of these EII genes, they were inactivated via homologous recombination and the resulting mutants characterized for sugar utilization. Whereas the sucrose EII was the only transport system for sucrose in C. glutamicum, fructose and glucose were each transported by a second transporter in addition to their corresponding EII. In addition, the ptsF ptsG double mutant carrying deletions in the EII genes for fructose and glucose accumulated fructose in the culture broth when growing on sucrose. As no fructokinase gene exists in the C. glutamicum genome, the fructokinase gene from Clostridium acetobutylicum was expressed in C. glutamicum and resulted in the direct phosphorylation of fructose without any fructose efflux. Accordingly, since fructokinase could direct fructose flux to the pentose phosphate pathway for the supply of NADPH, fructokinase expression may be a potential strategy for enhancing amino acid production.

ISSN 

0378-1097

Link 

http://dx.doi.org/10.1016/j.femsle.2005.01.053

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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