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Title 

Multichannel surface plasmon resonance imaging and analysis of micropatterned self-assembled monolayers and protein affinity interactions

Authors 

H B PyoYong Beom ShinMin-Gon KimH C Yoon

Publisher 

American Chemical Society

Issue Date 

2005

Citation 

Langmuir, vol. 21, no. 1, pp. 166-171

Keywords 

proteinsself assemblysurface plasmon resonancemultichannel imagingsurface plasmonsimaging techniquesproteinmonolayer

Abstract 

Multichannel images of 11-mercaptoundecanoic acid and 11-mercapto-1-undecanol self-assembled monolayers together with a biospecific interferon-γ (IFN-γ)/anti-IFN-γ antibody immunoaffinity interaction were observed by the two-dimensional surface plasmon resonance (2D-SPR) imaging system. With the fabricated 2D-SPR imaging system, adopting a white light source in combination with a narrow band-pass filter, sharp images were resolved, minimizing the diffraction patterns on the resulting images. Micropatterning of self-assembled monolayers was acheived by exploiting the UV photolysis of thiol bonding, instead of conventional photolithography. The line profile calibration of the image contrast with ellipsometric analysis enabled us to discriminate the change in monolayer thickness within a sub-nanometer scale. For the protein interactions on the surface, the biospecific affinity recognition reaction of IFN-γ antigen with surface-immobilized antibody was analyzed. Through the signal amplification strategy based on the enzymecatalyzed precipitation reaction in a sandwich-type immunoassay, biospecific antigen binding was found detectable down to a concentration of 1 ng/mL.

ISSN 

0743-7463

Link 

http://dx.doi.org/10.1021/la0486382

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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