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Title 

Enhanced sensitivity of surface plasmon resonance (SPR) immunoassays using a peroxidase-catalyzed precipitation reaction and its application to a protein microarray

Authors 

Min-Gon KimYong Beom ShinJin Mi JungH S RoBong Hyun Chung

Publisher 

Elsevier

Issue Date 

2005

Citation 

Journal of Immunological Methods, vol. 297, no. 1, pp. 125-132

Keywords 

horseradish peroxidaseimmunoassayinterferon-γinterleukin-5surface plasmon resonance (SPR)gamma interferonhuman interferonprocollagen c proteinasebiocatalyst

Abstract 

We describe a method to improve the sensitivity of surface plasmon resonance (SPR) immunoassays using a horseradish peroxidase (HRP)-catalyzed precipitation reaction. The precipitation reaction catalyzed by HRP bound to the SPR biosensor surface via a sandwich immunoassay induced a shift in the SPR angle. Human interferon (IFN)-γ at concentrations ranging from 0.01 to 100 ng/ml was detectable by this method. We also show that this biocatalytic signal amplification method can be applied to SPR imaging (SPRI), in an immunoassay of multiple proteins on a protein microarray format.

ISSN 

0022-1759

Link 

http://dx.doi.org/10.1016/j.jim.2004.12.007

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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